Overview

• Identify sites of lentiviral integration into the host genome

• Simple determination of lentivirus integration sites using GenomeWalker technology

• Compatible with most commercially available lentivirus vectors

• Complete system including all the necessary enzymes, primers, controls, PCR kit, genomic DNA purification kit, and gel purification kit

• Retroviral version also available for detecting integration sites for MMLV and MSCV retroviruses

• Identify sites of lentiviral integration into the host genome 

Product Description

Identify the exact site of integration of recombinant lentiviral vectors using the Lenti-X Integration Site Analysis Kit. The replication life cycle of a lentivirus results in integration of a DNA copy of the lentiviral RNA genome into the host genome. The chosen integration site has important consequences for both the expression of your transgene and the phenotype of the host cell.

Substance

Lenti-X Integration Site Analysis Kit (Product code 631263)

• Dra I
• 10× Dra I Restriction Buffer
• Ssp I
• 10× Ssp I Restriction Buffer
• Hpa I
• 10× Hpa I Restriction Buffer
• Control Human Genomic DNA (Lentivirus; 0.1 μg/μl)
• T4 DNA Ligase
• 10× Ligation Buffer
• GenomeWalker Adaptor
• Adaptor Primer 1
• Nested Adaptor Primer 2
• Lentivirus Specific Primer 1
• Nested Lentivirus Specific Primer 2
• NucleoSpin Tissue (Product Code 740952.10)
• NucleoSpin Gel and PCR Clean-up (Product Code 740609.50)
• Advantage 2 PCR Kit (Product Code 639207)

Preservation

-20°C

Note:
NucleoSpin Tissue and NucleoSpin Gel and PCR Clean-up should be stored at room temperature.

Visit Takara Bio website : https://www.takarabio.com/products/gene-function/viral-transduction/lentivirus/integration-site-analysis?catalog=631263